School of Aquatic Sciences and Fisheries Technology (SoAF)

Permanent URI for this community

https://repository.udsm.ac.tz/handle/20.500.11810/5553
School of Aquatic Sciences and Fisheries Technology, formerly department of Aquatic Sciences and Fisheries in the College of Agriculture and Fisheries (CoAF)

Browse

Browsing School of Aquatic Sciences and Fisheries Technology (SoAF) by Author "Changle Qi"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Dietary magnesium improves growth, antioxidant capacity, ammonia tolerance and intestinal health of juvenile freshwater prawn, Macrobrachium nipponense
    (Elsevier, 2022-11-15) Youqin Kong; Zhili Ding; Shanshan Wei; Changle Qi; Yan Liu; Yixiang Zhang; Samwel Mchele Limbu; Jinyun Ye
    The present study evaluated the effects of magnesium (Mg) on growth, antioxidant capacity, intestinal morphology and ammonia nitrogen resistance in juvenile oriental river prawn, Macrobrachium nipponense. Six semipurified diets (crude protein 401.1 g kg−1 diet; crude lipid 88.7 g kg−1 diet) containing Mg levels of 1.1, 1.4, 1.6, 2.1, 2.8 or 4.5 g kg−1 were formulated. A total of 1080 healthy juvenile prawns (0.151 ± 0.003 g) were randomly stocked into eighteen 300 L tanks (100 × 80 × 60 cm), each tank with 60 prawns. Each experimental diet was fed to prawns in triplicate twice daily (08:00 and 17:00) for eight weeks. After the feeding trial, 20 prawns from each tank were challenged with 37 mg/L ammonia nitrogen for 96 h, and the hemolymph, hepatopancreas and muscle of the rest of the prawns were collected. Final biomass was higher in prawns fed diets containing 1.6 and 2.1 g Mg kg−1 compared with those fed the diet with 1.1 g kg−1. Higher alkaline phosphatase (ALP) activity was obtained in the serum of prawns fed the 2.1 and 2.8 g Mg kg−1 diets. The highest activities of hepatopancreas superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) were observed in prawns fed 1.6–2.8 g Mg kg−1 of feed, and the lowest hepatopancreas malondialdehyde (MDA) concentrations and serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities were found in these treatment groups. Similarly, the prawns fed diets with 1.6 and 2.1 g Mg kg−1 had significantly lower MDA concentrations and cumulative mortality and higher antioxidant enzyme activities after ammonia nitrogen exposure. The intestinal epithelial cells were closely attached to the membrane in prawns fed the 2.1 g Mg kg−1 diet, but they were partly detached in those fed the 1.1 and 4.5 g Mg kg−1 diets. The minimum dietary Mg supplementations of juvenile M. nipponense were determined to be 1.6, 1.9, 2.0 and 2.2 g kg−1 feed, respectively, using broken-line regression analysis based on the final biomass, SOD, ALT and ALP.
  • Thumbnail Image
    Item
    Dietary mannan oligosaccharide (MOS) improves growth performance, antioxidant capacity, non-specific immunity and intestinal histology of juvenile Chinese mitten crabs (Eriocheir sinensis). Aquaculture, 510: 337–346.
    (Elsevier, 2019-08-15) Jian-Ting Lu; Changle Qi; Samwel Mchele Limbu; Fenglu Han; Jian Qin; Liqiao Chen; Lu Yang; Xiaodan Wang
    Mannan oligosaccharide (MOS) is a common prebiotics to improve health and immunity of animals in aquaculture. However, its optimum level and effects on the immune response in crab are currently unknown. The present study investigated the optimum level of dietary MOS supplementation and its effects on growth performance, antioxidant capacity, non-specific immunity and intestinal morphology of juvenile Chinese mitten crab (Eriocheir sinensis). Crab juveniles (2.95 ± 0.05 g) were fed either a control diet or one of the six diets supplemented with MOS (0.1%, 0.2%, 0.3%, 0.4%, 0.5% and 0.6%) for eight weeks. The crabs fed 0.3% MOS showed greater weight gain, specific growth rate and lower feed conversion ratio than those fed the control diet. The acid phosphatase activity was significantly higher in the gut of crab fed 0.2% MOS than those fed the other diets except the 0.3% MOS diet. The lysozyme and alkaline phosphatase activities in the gut were higher in crabs fed the 0.1% and 0.3% MOS diets compared to those fed other diets. The antioxidant capacity was higher in crab fed the 0.2% and 0.3% MOS diets than those fed other diets. In addition, the mRNA expression of genes related to immunity (E. sinensis (Es)-Crustin, Es-Toll2, Es-Lech and Es-prophenoloxidase (proPO)) in the hepatopancreas of crabs fed the 0.2 and 0.3% MOS diets were significantly up-regulated compared with those fed other diets. Duplicature length and width increased significantly in the crab fed 0.3% MOS than other diets. The optimum inclusion levels of MOS were 0.32%, 0.20% to 0.30% and 0.27% to 0.29% based on growth performance, antioxidant capacity and immunity, respectively. This study indicates that supplementing diets with MOS at 0.2% to 0.3% can improve growth performance and enhance antioxidant capacity and immunity in E. sinensis.