School of Aquatic Sciences and Fisheries Technology (SoAF)

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https://repository.udsm.ac.tz/handle/20.500.11810/5553
School of Aquatic Sciences and Fisheries Technology, formerly department of Aquatic Sciences and Fisheries in the College of Agriculture and Fisheries (CoAF)

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Browsing School of Aquatic Sciences and Fisheries Technology (SoAF) by Author "Baoliang Bi"

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    Arginine supplementation in plant-rich diets affects growth, feed utilization, body composition, blood biochemical indices and gene expressions of the TOR signaling pathway in juvenile Asian red-tailed catfish (Hemibagrus wyckoiides)
    (Wiley, 2022-02) Dahai Gu; Jingying Zhao; Samwel Mchele Limbu; Yunan Liang; Junming Deng; Baoliang Bi; Lingfu Kong; Hui Yan; Xiaowen Wang; Qing Hu; Yu Gao; Dan Jia; Hua Rong
    Arginine (Arg) is an important essential amino acid required for optimum fish growth. However, its role in plant-rich diets on growth and the required level in juvenile Hemibagrus wyckioides are currently unknown. This study evaluated the effects of Arg supplementation in plant-rich diets on growth, feed utilization, whole body composition, blood biochemical indicators and gene expressions of the target of rapamycin (TOR) signaling pathway in juvenile H. wyckioides. A total of 450 fish (6.40 ± 0.06 g) were randomized into six equal groups and fed on diets with graded Arg levels: 0 (control), 3, 6, 9, 12 and 15 g/kg of dry feed for eight weeks. The results revealed that specific growth rate (SGR) and feed conversion ratio of juvenile H. wyckioides were significantly improved and reduced, respectively in fish fed on 9 g/kg of Arg supplementation (p < .05). The optimum amount of dietary Arg content in plant-rich diets for H. wyckioides was 63.77 g/kg diet based on SGR. Feeding fish with dietary Arg supplementation ranging from 9 to 12 g/kg improved significantly (p < .05) whole body protein contents and decreased lipid deposition. The mRNA levels of TOR, eukaryotic translation initiation factor 4E (4EBP) and ribosomal protein S6 kinase 1 (S6K1) in muscle and liver were significantly affected by increasing dietary Arg levels. Meanwhile, the mRNA relative expression levels of TOR and S6K1 in the liver and muscle increased significantly as Arg supplementation increased. On the other hand, the mRNA relative expression levels of 4EBP was significantly higher in fish fed on the 9 g/kg Arg diet compared to those fed on the control and 15 g/kg Arg diets. In conclusion, our current results indicate that dietary Arg supplementation in plant-rich diets, might activate the TOR signaling pathway, which promotes protein synthesis and reduces lipid accumulation of H. wyckioides, resulting in improved growth.
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    Arginine supplementation in plant-rich diets affects growth, feed utilization, body composition, blood biochemical indices and gene expressions of the TOR signaling pathway in juvenile Asian red-tailed catfish (Hemibagrus wyckoiides), Journal of the World Aquaculture Society, 1–18. .
    (Wiley, 2020-10-12) Dahai Gu; Jingying Zhao; Samwel Mchele Limbu; Yunan Liang; Junming Deng; Baoliang Bi; Lingfu Kong; Hui Yan; Xiaowen Wang; Qing Hu; Yu Gao; Dan Jia; Hua Rong
    Arginine (Arg) is an important essential amino acid required for optimum fish growth. However, its role in plant‐rich diets on growth and the required level in juvenile Hemibagrus wyckioides are currently unknown. This study evaluated the effects of Arg supplementation in plant‐rich diets on growth, feed utilization, whole body composition, blood biochemical indicators and gene expressions of the target of rapamycin (TOR) signaling pathway in juvenile H. wyckioides. A total of 450 fish (6.40 ± 0.06 g) were randomized into six equal groups and fed on diets with graded Arg levels: 0 (control), 3, 6, 9, 12 and 15 g/kg of dry feed for eight weeks. The results revealed that specific growth rate (SGR) and feed conversion ratio of juvenile H. wyckioides were significantly improved and reduced, respectively in fish fed on 9 g/kg of Arg supplementation (p < .05). The optimum amount of dietary Arg content in plant‐rich diets for H. wyckioides was 63.77 g/kg diet based on SGR. Feeding fish with dietary Arg supplementation ranging from 9 to 12 g/kg improved significantly (p < .05) whole body protein contents and decreased lipid deposition. The mRNA levels of TOR, eukaryotic translation initiation factor 4E (4EBP) and ribosomal protein S6 kinase 1 (S6K1) in muscle and liver were significantly affected by increasing dietary Arg levels. Meanwhile, the mRNA relative expression levels of TOR and S6K1 in the liver and muscle increased significantly as Arg supplementation increased. On the other hand, the mRNA relative expression levels of 4EBP was significantly higher in fish fed on the 9 g/kg Arg diet compared to those fed on the control and 15 g/kg Arg diets. In conclusion, our current results indicate that dietary Arg supplementation in plant‐rich diets, might activate the TOR signaling pathway, which promotes protein synthesis and reduces lipid accumulation of H. wyckioides, resulting in improved growth.
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    Effects of dietary proline on swim bladder collagen synthesize and its possible regulation by the TGF-β/Smads pathway in Nibea diacanthus. Aquaculture Nutrition, 26(5): 1792-1805.
    (Wiley, 2020-10) Hua Rong; Fan Lin; Samwel Mchele Limbu; Zhideng Lin; Baoliang Bi; Tengfei Dou; Lei Zhao; Xiaobo Wen
    Swim bladder is an ideal source of collagen production in fish for improved human health. Proline (Pro) is the main proteinogenic amino acid needed for collagen production. However, the effects of Pro supplementation on the swim bladder collagen synthesis have rarely been evaluated in fish. We determined the effects of dietary Pro supplementation on swim bladder collagen synthesis and its possible signalling pathway in spotted drum, Nibea diacanthus. A total of 450 N. diacanthus (100 ± 3.05 g) were randomly assigned into six treatments and fed with diets supplemented with different levels of Pro (0, 2.5, 5, 7.5, 10 and 12.5 g/kg of dry diet, hereafter P0, P1, P2, P3, P4 and P5, respectively) for 8 weeks. At the end, we evaluated collagen synthesis in swim bladder and the expression of genes related to TGF‐β/Smad pathway in the fish. Dietary Pro levels increased significantly the contents of crude protein, total collagen (TC) and the levels of some amino acids in swim bladder than the control diet (p < .05). The optimum amount of dietary Pro inclusion in diets for swim bladder collagen synthesis in N. diacanthus was 7.6 and 7.5 g/kg based on crude protein and TC in swim bladder, respectively. Dietary Pro levels increased significantly the proline 4‐hydroxylase (P4H) content in fish serum, swim bladder, muscle and liver tissues than control (p < .05). The relative expression of collagen type I alpha 1 (COL1A1), alpha 2 (COL1A2) and mothers against decapentaplegic homolog 2 (Smad2) genes in liver and swim bladder initially increased significantly as the concentration of Pro and later decreased (p < .05). Similarly, the relative expression of transforming growth factor beta (TGF‐β), P4Ha2 and P4Ha3 genes in the swim bladder increased significantly as dietary Pro levels increased (p < .05). Using K‐means clustering analysis, dietary proline partly promoted collagen accumulation in swim bladder through upregulation of Smad2 and TGF‐βRT genes. Taken together, Pro affected the collagen metabolism in swim bladder, probably by regulating the TGF‐β/Smad pathway, most likely via transient overexpression of Smad2 gene.